Tag: ibotenic acid

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TGA Laboratory analysis for Calm Mushroom Resin Extract

Sample No63 concentrate

Visual description

Dense, easily deformable, non-sticky, specific odor, slightly oily

DSC– differential scanning calorimetry (change in heat flow with temperature)

This % defining analysis was previously used, but with increasing concentration the method is too sensitive, which is illustrated by many peaks at 140 ° C.

Therefore, TGA (thermogravimetric method) will hence be used, in which the change in mass of the material was determined. Compared to the previous method, the material was not damaged but converted to melted state by determining the melting point, but the TGA method is a material destruction method, thus the material gets damaged. Each substance has its own temperature at which the structure collapses or, more precisely, becomes not functional, making it possible to determine the mass fraction of each substance in the material from the total mass that was initially introduced.

TGA: After 1st derivative (1 / min) the amount of substances present in 5-7 components can be seen.

The question here is, have you used any additives, or is there a possibility of any substance entering the press? Given that the largest amount in the composition should be muscimol, the largest peak appears from 536.97 ° C to 695.08 ° C, and using the law of mass loss, we can calculate each component (see Table 1), but the device offers the possibility to determine immediately in this range how much weight is lost, which is the loss of muscimol, respectively (8.5%). The temperature of destruction is not mentioned anywhere in the scientific literature, so an actual hypothesis has been put forward here, to the exclusion of other variants. The TGA method has a limited ability to determine the destruction temperatures of the other components because there is no theoretical information that can overlap.

Muscimol

NAME:Muscimol
CHEMICAL NAME:5- (Aminomethyl) -3 (2H) -isoxazolone
ALTERNATE CHEMICAL NAMES:5- (aminomethyl) -3-isoxazolol, 5-aminomethyl-3-hydroxyisaxazole
ALTERNATE CHEMICAL NAMES:3-hydroxy-5-aminomethylisoxazole; agarin, pantherine
CHEMICAL FORMULAC4H6N2O2
MELTING POINT175 ° (crystals) (DSC)
DESTRUCTION TEMPERATURE600 ° C (Information obtained by us)

Given the possible presence of ibotenic acid in the DSC data (see Figure 3), its assumption may be negligible if the correct acquisition conditions were observed.

Ibotenic acid

NAME:Ibotenic Acid
CHEMICAL NAME:alpha-Amino-2,3-dihydro-3-oxo-5-iso-xazoleacetic acid
ALTERNATE CHEMICAL NAMES:alpha-amino-3-hydroxy-5-isoxazoleacetic acid
ALTERNATE CHEMICAL NAMES:amino- (3-hydroxy-5-isoxazolyl) acetic acid
CHEMICAL FORMULAC5H6N2O4
MOLECULAR WEIGHT158.11
MELTING POINT151-152 ° (anhydrous crystals)
MELTING POINT144-146 ° (monohydrate crystals)
DSC peaks characterizing the possible presence of ibotenic acid and muscasone. Range 139.70 – 157.16 ° C, peak 153 ° C

Muscatone

NAME:Muscazone
CHEMICAL NAME:alpha-Amino-2,3-dihydro-2-oxo-5-oxazoleacetic acid
ALTERNATE CHEMICAL NAMES:alpha-amino-2-oxo-4-oxazoline-5-acetic acid
CHEMICAL FORMULAC5H6N2O4
MOLECULAR WEIGHT158.11

As such, there are many different other substances in mushrooms that qualify as nutrients: vitamins A; B1B2; C; D1, PP. Mushrooms also contain minerals: potassium, phosphorus, iron; trace elements: zinc, copper, magnesium, iodine. Mushrooms are also rich in extracts. Of course, in the case of fly agaric, it is worth mentioning the pigment that gives red color. This means that both valuable substances and muscimol are ingested at the same time when consuming such a product.

Conclusion: Muscimol content 8.5% in a specific sample No63 concentrate.

2/14/2022

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Calm Mushroom extract – the scientific approach

Our Calm Mushroom Amanita Muscaria Standardized Water-Based Extract contains a standard content of active substances. The extensive research we’ve done into Amanita Muscaria helped us perfect the extract creation process. During the process all the useful polysaccharides and substances are freed from chitin and stored in the extract, while the temperature during dehydration and extraction does not exceed 40° C, ensuring the high quality of the end product.

Calm Mushroom extract

To obtain 10 grams of fly agaric resin extract, 50 grams of dried mushrooms are used.  To obtain 50 grams of dried mushrooms, at least 600 grams of high-quality fresh fly agaric caps (1:12) are used.  Therefore, to obtain 1 gram of fly agaric resin, 60 grams of fresh mushrooms are needed.  But the problem is that mushrooms differ in the content of active substances. This depends on the place of collection, dehydration technology, storage time and many other factors. The weight of the mushroom is important, but it is most important to obtain a standard content of active substances in the extract.  The main substance in the body of the fungus is chitin. It is useless for our body. We use a special technology to extract useful substances into purified water. The main substance is muscimol.  Important polysaccharides and vitamins are also preserved in the extract, because the cooking temperature does not exceed 40°C. 

Amanita Muscaria mushrooms

Quality of Calm Mushroom extract is proven by the analysis made in the scientific laboratory of Latvian University using the Gas Chromatography Mass Spectrometry (GC-MS) method. Analysis showed that our Amanita Muscaria extract contains high % of muscimol – 14.5% to be specific and 0% harmful neurotoxic ibotenic acid, making it 100% ibotenic acid free.

Ibotenic Acid chemical structure

By conducting multiple extraction experiments and performing dozens of analysis in the laboratory of the University, we became confident in the quality and content of the main active substance in our product – muscimol.  

Muscimol chemical structure

The research was conducted with the help of scientists, chemists, doctors and pharmacists from Europe and Canada. As a result, a technology for the industrial production of a standardized water extract of Amanita Muscaria – Calm Mushroom Standardized Water-Based Extract was created.  

In essence Calm Mushroom Extract is made of 10% Amanita Muscaria resin dissolved in vegetable glycerin and water. Because of muscimol, its main active ingredient, the resulting product has various therapeutic effects.

When applied to the skin it relieves pain, while a few drops under the tongue held for 1-2 minutes are enough to relieve anxiety. The extract also relieves hangxiety in 3-5 min and helps with insomnia. 

Apart form being ibotenic acid free Calm Mushroom Standardized Water-Based Extract also does not contain alcohol, since it is purely water based extract. Moreover our extract has a standard content of muscimol and is made entirely from natural plant substances.